Artificial intelligence (AI) strategies are exhibiting a shift of perspectives, focusing more intensively on a more human-centric view. New conceptualizations of AI literacy (AIL) are being presented, summarizing the competencies human users need to successfully interact with AI-based systems. However, these conceptualizations lack practical relevance. In view of the rapid pace of technological development, this contribution addresses the urgent need to bridge the gap between theoretical concepts of AIL and practical requirements of working environments. It transfers current conceptualizations and new principles of a more human-centered perspective on AI into professional working environments. From a psychological perspective, the project focuses on emotional-motivational, eudaimonic, and social aspects. Methodologically, the project presented develops AI testbeds in virtual reality to realize literally graspable interactions with AI-based technologies in the actual work environment. Overall, the project aims to increase the competencies and the willingness to successfully master the challenges of the digitalized world of work.