A growing emphasis on well-being in technology development raises the need for adequate measurement methods to quantify technology’s influence on individuals’ well-being. Psychological research has identified different well-being orientations, including hedonia (seeking comfort, relaxation, and pleasure) or eudaimonia, which emphasizes personal growth, excellence, meaningfulness, and authenticity. In particular, promoting eudaimonic well-being (EWB) continues to be a challenge in human-computer interaction as it manifests itself as a multidimensional construct. This paper presents the Eudaimonic Interaction Inventory, a scale for quantifying the experience of four core aspects of eudaimonic virtues (authenticity, meaning, excellence, growth) in interaction with technology. The inventory was validated through six steps across three distinct studies, resulting in twelve items categorized into four subscales. With this inventory, we hope to contribute to EWB research in technology by making future interactions with technology measurable in terms of EWB.